The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation in Bulk

Step-by-step protocol for the use of the Vybrant® MTT Cell Proliferation Assay Kit to quantify cell numbers based on metabolic activity in microplates

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mtt stock Final concentration of MTT is mgml • Use 1xPBS to dissolve the MTT powder • Filter it before use Procedure 1 After incubation of cells